Microbial Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on microbial cells, particularly eukaryotic microbes and bacteria, as well as plasmids and bacteriophages. * * Methods presented for ease of use and ready adaptation to new systems. * Detailed protocols included for: * Eukaryotic microbes - protozoan parasites (forward and reverse genetics, genome analysis), filamentous fungi (chromosome and gene analysis) * Yeast chromosomes - YACs, genome mapping, transcription factors, nucleosomes, recombination, RNA polymerase, pheromones. * Bacterial gene structure and regulation - E. coli (DNA methylation, mRNA characterization, gene regulation), B Subtilis (genetic mapping, chemotaxis), computer identification of genes. * Plasmids and bacteriophages - plasmid templates for transcription assays, plasmid replication: bacteriophage transcription, molecular genetic analysis using phages, phage assembly
Molecular Genetics of Eukaryotic Microbes: Forward and Reverse Genetics in the Study of the Obligate, Intracellular Parasite Toxoplasma Gondii. Techniques Associated with Protozoan Parasite Genome Analysis. Aspergillus Nidulans as a Model System for Cell and Molecular Biology Studies. Special Molecular Techniques for the Study of Filamentous Fungi. Yeast Vhromosomes: Transcription, Recombination, Replication: In Vitro Fragmentation of Yeast Chromosomes and Yacs at Artificially Inserted Sites and Applications to Genome Mapping. Basic Analysis of Transcription Factor Binding to Nucleosomes. Experimental Analysis of Transcription Factor: Nucleosome Interactions. In Vivo Analysis of Nucleosome Structure and Transcription Factor Binding in Saccharomyces Cerevisiae. Mapping of Yeast Nucleosome in Vivo. Novel Assays for Ligation and Cleavage Activities of Site-Specific Recombinases. Physical Monitoring of Mitotic and Meiotic Recombination in Saccharomyces Cerevisiae. Genetic Dissection of Complex Heteromultimeric Enzymes: The Case of Yeast DNA-Dependent RNA Polymerases. Pheromone Procedures in Fission Yeast. Bacterial Gene Structure and Regulation: Use of DNA Methylation Deficient Strains in Molecular Genetics. Identifying the Ends of Infrequent RNA Molecules in Bacteria. Identification of Rcs Genes in Escherichia Coli 09:K30:H12 and Their Involvement in Regulation of Expression of the Group IAK30 Capsular Polysaccharide. Genetic Mapping in Bacillus Subtilis. Cloning of Thermomonospora Fusca Cellulase Genes in Escherichia Coli and Streptomyces Lividans. Computational Analyses Aiding Identification and Characterization of Protein, Genes, And Operons. Plasmids and Phages: Replication, Transcription, Assembly: Techniques to Isolate Specialized Templates for in Vitro Transcription Assays. Protein-Protein Interactions of DNA-Binding Protiens: Studies in the Replication Initiator Protein, Rap A, Of Plasmid P1. Transcriptional Regulators: Protein Dna Complexes and Regulatory Mechanisms. Use of Coliphage Lambda and Other Bacteriophages for Molecular Genetics Analysis of Erwinia and Related Gram-Negative Bacteria. Isolation, Purification and Function of the Assembly Intermediates and Subviral Particles of Bacteriophages PRD 1 and O6. Index
