MARC 主機 00000nam a2200481K 4500
001 AAI27731903
005 20200821052220.5
006 m o d
007 cr mn ---uuuuu
008 200821s2019 miu sbm 000 0 eng d
020 9781392441213
035 (MiAaPQ)AAI27731903
035 (MiAaPQ)NCState_Univ18402037147
040 MiAaPQ|beng|cMiAaPQ|dNTU
100 1 Dangi, Saroj
245 10 Nanoplumbing for a Single Molecule Study of DNA
264 0 |c2019
300 1 online resource (132 pages)
336 text|btxt|2rdacontent
337 computer|bc|2rdamedia
338 online resource|bcr|2rdacarrier
500 Source: Dissertations Abstracts International, Volume: 81-
07, Section: B
500 Advisor: Daniele, Michael;Thomas, John;Wang, Hong;Riehn,
Robert
502 Thesis (Ph.D.)--North Carolina State University, 2019
504 Includes bibliographical references
520 Advancements in single molecule methods have paved a way
to understand the details of biomolecular interactions
with ever higher temporal and spatial resolution.
Nanofluidics is one of the key techniques for single
molecule biophysics with applications expanding to the
field of biotechnology such as gene mapping, DNA sorting,
and biosensing. In this work two different avenues of
nanofluidics are explored. First, a nanofluidic
metamaterial is designed and studied for the manipulation
of transport of DNA through confined space. The
manipulation is achieved by a combination of asymmetric
nanochannels and junctions, which can be tuned with buffer
flow. To understand the experimental observations of the
transport of DNA through an asymmetric junction, a two-
dimensional mean-field model of DNA at the junction is
proposed. The nonequilibrium dynamics of confined DNA at
the junction is probed with the proposed model and
compared with the experimental results. Second, a
combinatorial nanofluidic device is designed for the
single molecule experiment of DNA-protein interactions.
The device utilizes nanochannels, nanoslits, and
nanogrooves for the confinement of DNA and active buffer
exchange. The functionalities of the device for dynamic
manipulation of DNA and combinatorial exchange of buffers
in real-time are demonstrated with various experiments,
such as dye diffusion, conformational changes of DNA with
different ionic concentration, and restriction mapping of
lambda DNA. Overall, the results of this thesis show that
the combination of various nanofluidic geometries enables
to design a novel nanofludic device for the manipulation
of biomolecules at the single molecule level
533 Electronic reproduction.|bAnn Arbor, Mich. :|cProQuest,
|d2020
538 Mode of access: World Wide Web
650 4 Molecular physics
650 4 Applied physics
650 4 Nanotechnology
650 4 Bioinformatics
653 Nanofluidics
653 Biomoleculars
655 7 Electronic books.|2local
690 0652
690 0215
690 0715
690 0609
710 2 ProQuest Information and Learning Co
710 2 North Carolina State University
773 0 |tDissertations Abstracts International|g81-07B
856 40 |uhttps://pqdd.sinica.edu.tw/twdaoapp/servlet/
advanced?query=27731903|zclick for full text (PQDT)
912 PQDT
