MARC 主機 00000nam 2200000 a 4500
001 AAI3088632
005 20041014084349.5
008 041014s2003 s eng d
035 (UnM)AAI3088632
040 UnM|cUnM
100 1 Banatao, Diosdado Rey
245 10 Exploration of functional sites in complex RNA folds and
macromolecular assemblies|h[electronic resource]
300 158 p
500 Source: Dissertation Abstracts International, Volume: 64-
04, Section: B, page: 1615
502 Thesis (Ph.D.)--University of California, San Francisco,
2003
520 Structural genomics efforts enable the high-throughput
discovery of novel molecular structures and are quickly
outpacing traditional biochemical methods for determining
the function of those molecules. This flood of structural
data has created the need for rapid and automated ways to
annotate function to molecular structures. Additionally,
the interest in RNA structure and function has increased
rapidly within the past several years, as dramatic
discoveries have been made about novel biological
mechanisms directly involving RNA. The folding, structure,
and function of all RNA molecules are dependent on the
direct and indirect coordination of magnesium (Mg<super>2+
</super>) ions. However, locating Mg<super>2+</super>
binding sites is difficult experimentally and
computationally. Thus, in my doctoral research, I have
created and applied computational tools to address issues
in the identification of functional sites in RNA
structures and assemblies. I developed a novel approach,
based on a previous method of identifying functional sites
in protein structures, for understanding and locating Mg<
super>2+</super> binding sites in RNA. I have developed
additional tools for the integrated analysis and
visualization of my results with RNA structure. I have
also contributed to the development of a generally
applicable tool for knowledge modeling and structural
modeling of the ribosome, the large assembly of RNA and
proteins responsible for protein synthesis in the cell. I
have successfully validated this approach on
experimentally characterized RNA systems and have
demonstrated that it is scalable to the analysis of large
RNA assemblies. Most importantly, I have identified novel
Mg<super>2+</super> binding sites that may have important
functional roles in RNA catalysis, especially in ribosomal
activity. I provided detailed descriptions of these sites
that may guide experimentalists in the comprehensive
analysis of Mg<super>2+</super> binding in RNA and the
ribosome. My methods and discoveries may serve as tools
for rational drug design targeting RNA and its assemblies
590 School code: 0034
650 4 Biology, Molecular
650 4 Biophysics, General
650 4 Computer Science
650 4 Health Sciences, Pharmacology
690 0307
690 0786
690 0984
690 0419
710 20 University of California, San Francisco
773 0 |tDissertation Abstracts International|g64-04B
856 40 |uhttp://pqdd.sinica.edu.tw/twdaoapp/servlet/
advanced?query=3088632
912 PQDT
