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001 AAI28152727
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008 210823s2020 xx sbm 000 0 eng d
020 9798691247002
035 (MiAaPQ)AAI28152727
040 MiAaPQ|beng|cMiAaPQ|dNTU
100 1 Frith, Ashley
245 10 Environmental Effects on Histamine Production in
Decomposing Fish
264 0 |c2020
300 1 online resource (96 pages)
336 text|btxt|2rdacontent
337 computer|bc|2rdamedia
338 online resource|bcr|2rdacarrier
500 Source: Masters Abstracts International, Volume: 82-05
500 Advisor: Carmichael, Ruth H
502 Thesis (M.S.)--University of South Alabama, 2020
504 Includes bibliographical references
520 Histamine fish poisoning (HFP) is a common seafood-borne
illness caused by human consumption of fish containing
high concentrations of histamine. Illness is attributed to
histamine-producing bacteria (HPB) that proliferate in
fish muscles during storage. While individual strains of
HPB have been studied and their histamine-producing
capabilities determined, less is known about natural
communities of HPB. This study used 16S rDNA analysis and
a controlled decomposition study to determine factors that
affect the concentration and species of HPB in the
environment (Ch. 1), the proximate origin of HPB to fish
tissues (Ch. 2), and if either HPB or histamine
concentrations in decomposing fish varied among fish
sections (Ch. 3). I found that temperature and salinity
mediated the concentration and species composition of HPB
in the environment. Water was a source of HPB, but gills
and gut were more likely proximate sources to fish muscle.
HPB counts determined histamine concentrations in
decomposing fish muscle once HPB reached a threshold 2.3
log MPN g-1, and both varied with incubation time and
temperature and were greater in anterior fish sections.
These results add to our understanding of natural HPB
communities and can be used in a risk-assessment framework
to prevent outbreaks of HFP
533 Electronic reproduction.|bAnn Arbor, Mich. :|cProQuest,
|d2021
538 Mode of access: World Wide Web
650 4 Aquatic sciences
650 4 Microbiology
650 4 Food science
650 4 Limnology
650 4 Histology
653 16S
653 Bacteria
653 Decomposing fish
653 Histamine fish poisoning
653 Histidine decarboxylase
653 Real-time PCR
655 7 Electronic books.|2local
690 0792
690 0410
690 0359
690 0793
690 0414
710 2 ProQuest Information and Learning Co
710 2 University of South Alabama.|bMarine Sciences
773 0 |tMasters Abstracts International|g82-05
856 40 |uhttps://pqdd.sinica.edu.tw/twdaoapp/servlet/
advanced?query=28152727|zclick for full text (PQDT)
912 圖書館PQDT110|b1110406
