MARC 主機 00000nam a2200493 i 4500
001 978-981-15-4423-1
003 DE-He213
005 20200923100120.0
006 m o d
007 cr nn 008maaau
008 200509s2020 si s 0 eng d
020 9789811544231|q(electronic bk.)
020 9789811544224|q(paper)
024 7 10.1007/978-981-15-4423-1|2doi
040 GP|cGP|erda
041 0 eng
050 4 QP624.75.P74|bY898 2020
072 7 TCB|2bicssc
072 7 SCI010000|2bisacsh
072 7 TCB|2thema
082 04 572.86|223
100 1 YU, Zutao,|eauthor
245 10 Artificial assemblies with cooperative DNA recognition /
|cby Zutao YU
264 1 Singapore :|bSpringer Singapore :|bImprint: Springer,
|c2020
300 1 online resource (xv, 136 pages) :|billustrations,
digital ;|c24 cm
336 text|btxt|2rdacontent
337 computer|bc|2rdamedia
338 online resource|bcr|2rdacarrier
347 text file|bPDF|2rda
490 1 Springer theses,|x2190-5053
505 0 1. Synthetic transcription factors (Syn-TFs): design,
progress and perspectives -- 2. Pip-HoGu, an artificial
assembly with cooperative DNA recognition capable of
mimicking transcription factor pairs -- 3. Orthogonal γPNA
dimerization domains empower DNA binders with
cooperativity and versatility mimicking that of the
transcription factor pairs -- 4. Advanced DNA binding
system mimicking the cooperative function of transcription
factor pairs precisely recruits the epigenetic modifiers
to the DNA repeat binding sites
520 This book presents three types of synthetically
cooperative DNA recognizing assemblies, in order to
advance the development of programmable DNA-binding
pyrrole-imidazole polyamides (PIPs) PIPs represent the
best-characterized class of small molecule DNA binders
that can be modified to bind with any predetermined DNA
sequence and regulate gene expression patterns in a
transgene-free and cost-effective manner. PIPs are
characterized by their small molecular size, high binding
affinity, programmability, sequence selectivity, and
moderate cell permeability. In recent years, there have
been numerous novel studies on the applications of these
biological tools; this research is thoroughly reviewed in
the first chapter. There are several critical issues,
however, that impede the further broad study of PIPs,
which greatly concern the author. For instance, the short
PIP version has an excessively hî10 bp; this
significantly decreases cell permeability. Moreover, the
conventional binding strategy for PIP design cannot apply
to flexible DNA binding-for example, the DNA-binding mode
of a transcription factor pair. In this book, the author
describes the development of three kinds of cooperative
DNA-binding systems that help resolve the current highly
problematic issues concerning PIPs. These three systems
offer a range of significant advantages, such as favorable
sequence selectivity, long recognition sequence, higher
binding affinity, and a flexible gap distance. Released at
a critical juncture in the application of PIPs, this book
will greatly facilitate their use as therapeutic drugs in
the treatment of cancer and hereditary diseases, and in
regenerative medicine
650 0 DNA-binding proteins
650 0 Synthetic biology
650 14 Biotechnology
650 24 Bioorganic Chemistry
650 24 Nucleic Acid Chemistry
650 24 Medicinal Chemistry
710 2 SpringerLink (Online service)
773 0 |tSpringer eBooks
830 0 Springer theses
856 40 |uhttps://doi.org/10.1007/978-981-15-4423-1
912 Springer|b110906304615
