MARC 主機 00000cam  22000004a 4500 
001    s  00097065  
005    19990908100858.0 
008    050307s2006    enka     b    001 0 eng   
010    2005006606 
020    1405111216 (pbk. : alk. paper) 
020    9781405111218 (pbk. : alk. paper) 
040    DNLM/DLC|cDLC|dNLM|dBAKER|dUKM|dBWKUK|dEZU|dAGL|dOCLCQ
       |dDLC 
042    pcc 
050 00 QH442.2|b.B76 2006 
082 00 572.8/633|222 
100 1  Brown, T. A.|q(Terence A.) 
245 10 Gene cloning and DNA analysis :|ban introduction /|cT.A. 
       Brown 
250    5th ed 
260    Oxford ;|aMalden, MA :|bBlackwell Pub.,|c2006 
300    xx, 386 p. :|bill. ;|c25 cm 
504    Includes bibliographical references and index 
505 0  PART 1: THE BASIC PRINCIPLES OF GENE CLONING AND DNA 
       ANALYSIS: Why gene cloning and DNA are important -- 
       Vectors for gene cloning: plasmids and bacteriophages -- 
       Purification of DNA from living cells -- Manipulation of 
       purified DNA -- Introduction of DNA into living cells -- 
       Cloning vectors for E. coli -- Cloning vectors for 
       eukaryotes -- How to obtain a clone of a specific gene -- 
       The polymerase chain reaction -- PART 2: THE APPLICATION 
       OF GENE CLONING AND DNA ANALYSIS IN RESEARCH: Studying 
       gene location and structure -- Studying gene expression 
       and function -- Studying genomes -- PART 3: THE 
       APPLICATION OF GENE CLONING AND DNA ANALYSIS IN 
       BIOTECHNOLOGY: Production of protein from cloned genes -- 
       Gene cloning and DNA analysis in medicine -- Gene cloning 
       and DNA analysis in agriculture -- Gene cloning and DNA 
       analysis in forensic science and archaeology -- Glossary 
650  0 Molecular cloning 
650  0 Nucleotide sequence 
650  0 DNA|xAnalysis 
650 12 Cloning, Molecular 
650 22 DNA, Recombinant|xanalysis 
650 22 Sequence Analysis, DNA 
856 41 |3Table of contents|uhttp://www.loc.gov/catdir/toc/ecip059
       /2005006606.html 
856 42 |3Contributor biographical information|uhttp://www.loc.gov
       /catdir/enhancements/fy0802/2005006606-b.html 
856 42 |3Publisher description|uhttp://www.loc.gov/catdir/
       enhancements/fy0802/2005006606-d.html 
911    WJ/hml 
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